How to test AeT efficiency

I felt more muscle fatigue, but it was also true that I rode 3.5 hours before the test (light effort, but still on saddle) + it was the end of the day and on I didn’t completely recovered from a hard workout made on Saturday (Feedback about "Collywobbles 135 + CAD" CJ workout) that I made for about 1-1.5H at very high cadence (107 rpm).

Normally those tests should be made after very good recover, it wasn’t exactly the case this time.

What do you think about my question on how glycogen is used?

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